Automated Method for Determination of Bound N-Acetylneuraminic Acid in Serum
نویسندگان
چکیده
منابع مشابه
Improved automated determination of bound N-acetylneuraminic acid in serum.
The method in which thiobarbituric acid is used for sialic acid determination was adapted to a fully automated procedure, in which the chromogen need not be extracted with an organic solvent. The entire procedure, including hydrolysis, requires 35 mm and 25 l of serum for one analysis, but 30 samples can be analyzed per hour. The procedure, slightly modified, can be used to measure as little as...
متن کاملAutomated method for determination of bound N-acetylneuraminic acid in serum.
The usefulness of determining protein-bound sialic acid in serum is obvious,because a significant increase in various serum sialoglycoprotein concentrations (haptoglobin, orosomucoid, a1-antitrypsin, and ceruloplasmin) has been observed in many pathological states (1, 2). We earlier described(3) a fully automated method in which thiobarbitunc acid is used as a reagent.This very sensitive method...
متن کاملSialic Acid (n-acetylneuraminic Acid) in Human Serum.
A simple spectrophotometric method is described for the sialic acids which utilizes the reaction with resorcinol and extraction with butyl acetate-n-butanol as a solvent. Normal values for adults, utilizing N-acetylneuraminic acid as a standard, were found to be 60 ± 10.4 mg./100 ml. Values for newborn babies averaged 40.4 ± 5.7. Similar values were found for specimens from 2-month-old infants ...
متن کاملSynthesis of N-acetylneuraminic acid and of CMP-N-acetylneuraminic acid in the rat liver cell.
Adult male rats, under starving and normal conditions, were injected intravenously with N-acetyl[3H]mannosamine and after various time intervals the specific radioactivities of free N-acetylneuraminic acid (NeuAc) and CMP-N-acetylneuraminic acid were determined in the liver. The specific radioactivity of free NeuAc was high even within 20s after injection; the maximum was reached between 7 and ...
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A method for the determination of both direct and indirect serum bilirubin on the AutoAnalyzer is described which has a number of advantages over the automated method that is currently recommended. It gives total bilirubin results which are similar to those obtained with the equivalent manual method and direct bilirubin results which are usually about 10% lower.The effect of haemolysis on estim...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1975
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/21.3.412